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KMID : 0043320150380091651
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Archives of Pharmacal Research
2015 Volume.38 No. 9 p.1651 ~ p.1660
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Chemical biology of compounds obtained from screening using disease models
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Tashiro Estu
Imoto Masaya
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Abstract
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Bioactive compounds are extremely powerful tools for studying biological systems because they can rapidly, conditionally, often reversibly, and dose-dependently modulate the biological function of living cells. Moreover, they are expected to be drug seeds for chemotherapy of several diseases. Two approaches are used to find and obtain bioactive compounds, namely, molecular-target-based screening and phenotypic screening. Through phenotypic screening that mimics tumor metastasis, multi-drug resistance, and Parkinson’s disease, we identified several compounds that inhibit cancer cell migration, anti-apoptotic function of Bcl-2/Bcl-xL, and neuronal cell death. By using MEK inhibitor that was developed by target-based screening, we discovered that MEK inhibitor selectively induces apoptosis in tumor cells with β-catenin mutation. Using target-based screening, we identified arabilin, a novel androgen antagonist. In this review, we introduce our recent studies on the identification of bioactive compounds by phenotypic screening and by target-based screening for drug-seed discovery.
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KEYWORD
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Cell migration, Apoptosis, Parkinson’s disease, MEK, β-Catenin, Androgen
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